To raise the funds, CVS put customer and staff collection boxes in its small animal first opinion practices and referral hospitals.
Staff also held a number of fundraising events – including sponsored walks and dog shows, as well as hosting Pet Blood Bank donation days.
The money raised from the partnership will help go towards funding Pet Blood Bank’s work.
Last year the charity sent over 5,000 units of blood to vets across the UK.
Pet Blood Bank provides training for vets and nurses, and donations also help the charity to keep the cost of blood products as low as possible, and provide them free to veterinary charities.
Deputy Chief Executive of CVS Group, Ben Jacklin, said: “Pet Blood Bank is a vital charity doing crucial work within our profession.
"There is no doubt that the level of funds raised is down to the tireless commitment shown by our colleagues up and down the country.
"I’d also like to recognise the role our clients have also made - as without them we would never have reached such a great total.”
Managing Director of Pet Blood Bank, Katrina Wilkinson said: “This generous donation from CVS Group has been incredible to receive.
"We are a small charity so need all the support we can get, and CVS has truly delivered."
CVS has recently announced its next charity partner as Guide Dogs for the Blind.
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